An evaluation of human lymphocyte nuclear RNA with acridine orange.

with Acridine Orange By DAvID M. JOHNSON, PEYTON T. PIiArr AND PEnnY G. RuGBY I HE IMPORTANCE OF lymphocytic RNA in immune function has received widespread attention in recent years.’ A case in point is the work of Mannick and Egdahl showing that adoptive immunity can be transferred from one animal to another by means of lymphocytic RNA.2 The RNA of the lymphocyte can be evaluated and compared with the DNA by selective staining with acridine orange for fluorescent microscopy. 5 Therefore, a morphologic investigation of human lymphocytic RNA has been undertaken in normal and diseased individuals.